Respiratory signal calibration
This article details how to use the solution software's calibration module to convert your data from a.u. to ml
1. Why calibrate your respiratory data ?
The DECRO® system provides 3 biosignals, yet the Respiratory signal is given in an arbitrary unit, due to the plethysmography method. It is possible to perform a calibration, in order to transform the relative unit (arb.u) into an absolute value (ml).
There are three ways to calibrate data :
1. The first one is the AUTOCALIBRATION , a fast and simple calibration of the RIP signal which requiered the weight of the animals and a zone of the signal where the animal's state is considered basal, ideally at rest and calm, previously marked with a timeframe. This method is based on the literature (Association of Inhalation Toxicologists (AIT) Inhalation Guidelines).
2. The second one is the a calibration RIP signal based on a REFERENCE MEASURE wich requiered a reference tidal volume obtained
from a plethysmography box for example and the zone of the corresponding DECRO signal recorded in the plethysmography box where the animal's state is considered basal, ideally at rest and calm, previously marked with a timeframe.
3. The third one is the PREVIOUS CALIBRATION , which allows you to select a previous gain/calibration calculated using the previous methods.
2. How to perform a calibration in the software ?
To carry out a calibration on one of the acquisition sessions, click on the "CALIBRATION RIP" button just below the MERGE. (SESSION part of a study)
Then select the desired calibration method ...
A. Autocalibration
This calibration module is based on a reference state of the animal. It is necessary to have defined and positioned a timeframe on the MERGE sequence of the animal before performing the calibration.
The timeframe used for calibration must be placed at a time when the animal is NOT under handling or stress. A period of 30 min to 1 hour is recommended. This can include periods of spontaneous animal activity.
Once the timeframe has been positioned, click on CALIBRATE RIP and Click on "AUTO CALIBRATE"
Then fill in the following fields (Specify timeframe and weight) and click on AUTO CALIBRATE to validate
B. Reference measure calibration
It is necessary to have defined and positioned a timeframe on the MERGE sequence of the animal before performing the calibration.
The timeframe used for calibration must be placed at a time when the animal is measured to obtain the volume in ml by the external reference tool . A period of 30 min to 1 hour is recommended. This can include periods of spontaneous animal activity.
This calibration often involves a recording session with the DECRO tool and the external volume meter before recording your effect. Calibration is therefore performed on this recording session.
Once the timeframe has been positioned, for calibration by reference measurement, click on EXTERNAL TV MEASURMENT
Then simply enter the current volume and the corresponding timeframe you wish to use for calibration.
Finally click on CALIBRATE to validate
C. Previous calibration
The previous calibration shows the previous gain/calibration calculated using the previous method. It is possible to use this gain on another acquisition session
3. How to check whether calibration has been carried out?
The calibration is done when the RIP GAIN appears below the MERGE button and next to the CALIBRATION button:
The initially green CALIBRATE RIP button then turns orange and becomes RECALIBRATE RIP
It can also be viewed and modified in the calibration pop-up.
Once the calibration is done, the respiratory parameters (VT and MV) will be expressed in milliliter in the different views and exports of the software
Data related to respiratory volume in the Excel export of several recording sessions will be expressed in arb.u and ml.
Columns will be identified as follows
- VT_analysis = VT values in uncalibrated arb.u
- VT_cal_analyse = VT values in calibrated ml
The calibration gain for each individual is also specified (Columns RIP calibration)
Related Articles
How to manage the detection of physiological cycles? - Edit cycle mode.
The following article illustrates how to use the cycle editing feature. 1. What is the cycles management feature? During data processing, algorithms detect cardiac and respiratory cycles by placing event markers. Based on these, physiological ...Data analysis with LASA software (Last edition, Software version 2.16 )
This article provides the key steps for data analysis with Lasa software and data export to Excel. The Excel file containing the physiological variables calculated for several animals studied can be used to draw initial conclusions or to prepare the ...Data analysis with LASA software (first edition, software version 2.12)
This article provides the key steps for data analysis with Lasa software and data export to Excel. The Excel file containing the physiological variables calculated for several animals studied can be used to draw initial conclusions or to prepare the ...Study creation and deletion
In this article, you'll find out how to create, back-up and delete your study data. A) Study Creation 1- Study creation steps The creation of a new study is done from the home view of the software. Click on in order to create a new study. Fill in the ...ECG delineation analyzer - additional module
This article details how to use the solution software module to perform the ECG delineation. 1. What is the ECG delineation module? The system provides 3 biosignals: the initial cardiac parameters provided in the software by the ECG analyzer module ...