Respiratory signal calibration

Respiratory signal calibration

This article details how to use the solution software's calibration module to convert your data from a.u. to ml 

1. Why calibrate your respiratory data ? 

The DECRO® system provides 3 biosignals, yet the Respiratory signal is given in an arbitrary unit, due to the plethysmography method. It is possible to perform a calibration, in order to transform the relative unit (arb.u) into an absolute value (ml).





There are three ways to calibrate data :




The first one is the AUTOCALIBRATION , a fast and simple calibration of the RIP signal which requiered the weight of the animals and a zone of the signal where the animal is resting and calm, previously marked with a timeframe.

The second one is the PREVIOUS CALIBRATION , which allows you to select a previous gain/calibration calculated using the previous method.

The third one is the a calibration RIP signal based on a REFERENCE MEASURE wich requiered a reference tidal volume obtained from a plethysmography box for example and a zone of the signal where the animal is resting and calm, previously marked with a timeframe.

2. How to perform a calibration in the software ?

To carry out a calibration on one of the acquisition sessions, click on the "CALIBRATION RIP" button just below the MERGE. (SESSION part of a study)



Then select the desired calibration method ...

A. Autocalibration 

Click on "AUTO CALIBRATE"

Then fill in the following fields and click on  "AUTO CALIBRATE"

This calibration module is based on a reference state of the animal. It is necessary to have defined and positioned a "Baseline" timeframe (1) on the MERGE sequence of the animal before performing the calibration. 

The timeframe used for calibration must be placed at a time when the animal is NOT under handling or stress. A period of 30 min to 1 hour is recommended. This can include periods of spontaneous animal activity. 






The calibration is done when the gain appears : 


Once the calibration is done, the respiratory parameters (VT and MV) will be expressed in milliliter in the different views and exports of the software

B. Previous calibration 

The previous calibration shows the previous gain/calibration calculated using the previous method. It is possible to use this gain on another acquisition session

Once the calibration is done, the respiratory parameters (VT and MV) will be expressed in milliliter in the different views and exports of the software

C. Reference measure calibration

For calibration by reference measurement, simply enter the current volume and the time you wish to use for calibration (as for the AUTOCALIBRATION method, it is necessary to define a "Baseline" timeframe). 
Finally click on calibrate
Once the calibration is done, you will get another GAIN.


Once the calibration is done, the respiratory parameters (VT and MV) will be expressed in milliliter in the different views and exports of the software


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